Motsoaledi said the department of health had managed to acquire 1.3m doses of the H1N1 vaccine and another 3.5m doses from the World Health Organisation.
"We received a letter from the World Health Organisation that they are going to donate to South Africa 3.5m doses of H1N1 which will arrive in this country by March," he said, adding that the WHO donation saved South Africa about 250m rand (£20m).
With just over 100 days before the matches, the sector has to finalise preparations for responsive health services, as well as a disaster management plan and prompt responses.
The Limpopo Provincial 2010 FIFA Health Committee is pleased with moves to set up the centre as well as progress made towards the final preparations for the event.
The Committee, which met at the weekend, confirmed that Polokwane, Mankweng Hospital Complex, Lebowakgomo, Mokopane, Seshego, and Polokwane - Medi Clinic Hospital were ready for the World Cup.
Key facilities at these hospitals such as theatre operations, trauma centres and intensive care units have gone through an intensive checking process and will be ready to handle any casualty during the event.
Local organising committee (LOC) representative Victor Ramathasela said the country was ready to provide health services of world standard.
"I can confidently and boldly say that we are ready to provide health services of world standards and more."
Speaking during a panel discussion at the World Conference on Disasters and Emergency Medicine on Tuesday, Dr Wayne Smith, the head of the Western Cape's 2010 health planning unit, said it was "dangerous" for authorities to be over-confident in South Africa's medical ability to deal with disasters simply because the country hosted the Rugby and Cricket World Cups without incident.